Algologia 2017, 27(1): 15–21 https://doi.org/10.15407/alg27.01.015Physiology, Biochemistry, Biophysics
Effect of nitrogen on the accumulation of fucoxanthin from diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin.
Ryabushko V.I., Zheleznova S.N., Nekhoroshev M.V.- A.O. Kovalevsky Institute of Marine Biological Researches
- 2, Nakhimov Prosp., 299011 Sevastopol, Crimea
Abstract
The effect of inorganic nitrogen content in the culture medium on accumulation of fucoxanthin (Fc) in the diatom Cylindrotheca closterium was studied. The Fc content in the biomass of the microalgae is determined in the transition to the stationary growth phase. Increase of the concentration of nitrogen in the medium F/2 promotes accumulation of fucoxanthin in the culture. With the concentration of sodium nitrate 225–300 mg · L-1 in the medium, the concentration of fucoxanthin in dry biomass of C. closterium is found to reach 15 mg · g-1. The maximum cell concentration is above 2·106 cells · mL-1 and the average specific growth rate is 0.21 day-1. Use of nutrient medium with high concentration of nitrogen allows obtaining the culture of C. closterium enriched with the biologically active substance fucoxanthin. Consequently, the diatom Cylindrotheca closterium can be regarded as a promising object in biotechnology.
Keywords: Cylindrotheca closterium, cultivation, nitrogen, fucoxanthin
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